Glycosaminoglycans and proteoglycans of human bone tissue at different stages of mineralization.

@article{Engfeldt1976GlycosaminoglycansAP,
  title={Glycosaminoglycans and proteoglycans of human bone tissue at different stages of mineralization.},
  author={Bengt Engfeldt and Anders Hjerpe},
  journal={Acta pathologica et microbiologica Scandinavica. Section A, Pathology},
  year={1976},
  volume={84 1},
  pages={95-106}
}
Using ultracentrifugation, compact bone from a growing 15 years old man was separated into three fractions with different degrees of mineralization. The finely powdered material was fractionated in a density gradient of acetone and bromoform in varying proportions. The glycosaminoglycans were isolated after papain digestion of the tissue fractions. The glycosaminoglycan elution profiles from CPC-cellulose microcolumns indicated the presence of chondroitin-4-sulphate and, possibly, minor traces… CONTINUE READING