Glycosaminoglycan silencing by engineered CXCL12 variants.

  title={Glycosaminoglycan silencing by engineered CXCL12 variants.},
  author={Martha Gschwandtner and Martin U Trinker and Bianca Hecher and Tiziana Adage and Simi Ali and Andreas J. Kungl},
  journal={FEBS letters},
  volume={589 19 Pt B},
We have engineered GPCR (G protein-coupled receptor) knock-out and high GAG-binding affinity into CXCL12α to inhibit CXCL12α-induced cell migration. Compared to wtCXCL12, the mutant CXCL12α (Δ8 L29K V39K) exhibited a 5.6-fold and a 2.2-fold affinity increase for heparin and heparan sulfate, respectively. From NaCl-based heparin displacement chromatography we concluded that more amino acid replacements would lead to altered GAG (glycosaminoglycan) ligand specificity. GAG silencing by this mutant… CONTINUE READING
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