Glycoproteomic Analysis of the Secretome of Human Endothelial Cells*

  title={Glycoproteomic Analysis of the Secretome of Human Endothelial Cells*},
  author={X Cindy Yin and Marshall Bern and Qiuru Xing and Jenny Chung‐Yee Ho and Rosa Viner and Manuel Mayr},
  booktitle={Molecular & cellular proteomics : MCP},
Previous proteomics studies have partially unraveled the complexity of endothelial protein secretion but have not investigated glycosylation, a key modification of secreted and membrane proteins for cell communication. In this study, human umbilical vein endothelial cells were kept in serum-free medium before activation by phorbol-12-myristate-13 acetate, a commonly used secretagogue that induces exocytosis of endothelial vesicles. In addition to 123 secreted proteins, the secretome was… CONTINUE READING
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