Glycoprotein organization of Chikungunya virus particles revealed by X-ray crystallography

@article{Voss2010GlycoproteinOO,
  title={Glycoprotein organization of Chikungunya virus particles revealed by X-ray crystallography},
  author={James E. Voss and Marie Christine Vaney and St{\'e}phane Duquerroy and Clemens Vonrhein and Christine Girard-Blanc and Elodie Crublet and Andrew Thompson and Gerard Bricogne and F{\'e}lix A. Rey},
  journal={Nature},
  year={2010},
  volume={468},
  pages={709-712}
}
Chikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus that has caused widespread outbreaks of debilitating human disease in the past five years. CHIKV invasion of susceptible cells is mediated by two viral glycoproteins, E1 and E2, which carry the main antigenic determinants and form an icosahedral shell at the virion surface. Glycoprotein E2, derived from furin cleavage of the p62 precursor into E3 and E2, is responsible for receptor binding, and E1 for membrane fusion. In the… 
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References

SHOWING 1-10 OF 55 REFERENCES
Formation of the Semliki Forest virus membrane glycoprotein complexes in the infected cell.
TLDR
Proteolytic digestion of crude vesicle preparations derived from plasma membranes, combined with peptide mapping, indicate that the carboxy-terminal end of E2 spans the cell plasma membrane, there being a portion of mol.
The Disulfide Bonds in Glycoprotein E2 of Hepatitis C Virus Reveal the Tertiary Organization of the Molecule
TLDR
The resulting model of the tertiary organization of E2 gives key information on the antigenicity determinants of the virus, maps the receptor binding site to the interface of domains I and III, and provides insight into the nature of a putative fusogenic conformational change.
Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein
TLDR
Support is given for a model in which the E1 trimeric structure is involved in the SFV-mediated fusion reaction, and it is shown that the new oligomer is represented by an E1 trimmeder.
Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak
TLDR
The results indicate that the chikungunya virus outbreak was initiated by a strain related to East-African isolates, from which viral variants have evolved following a traceable microevolution history and may be due to adaptation to the mosquito vector.
A Single Mutation in Chikungunya Virus Affects Vector Specificity and Epidemic Potential
TLDR
The observation that a single amino acid substitution can influence vector specificity provides a plausible explanation of how this mutant virus caused an epidemic in a region lacking the typical vector, and has important implications with respect to how viruses may establish a transmission cycle when introduced into a new area.
Structural Changes of Envelope Proteins During Alphavirus Fusion
TLDR
The structure of an alphavirus spike, crystallized at low pH, representing an intermediate in the fusion process and clarifying the maturation process is reported.
Epistatic Roles of E2 Glycoprotein Mutations in Adaption of Chikungunya Virus to Aedes Albopictus and Ae. Aegypti Mosquitoes
TLDR
It is demonstrated that introduction of the E1-A226V mutation into the background of an infectious clone derived from the Ag41855 strain does not significantly increase infectivity for Ae.
...
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