Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.

Abstract

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that… (More)
DOI: 10.1021/ja2018477

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