Glycophospholipid membrane anchor attachment. Molecular analysis of the cleavage/attachment site.

@article{Moran1991GlycophospholipidMA,
  title={Glycophospholipid membrane anchor attachment. Molecular analysis of the cleavage/attachment site.},
  author={Paul Moran and Helga E Raab and William J. Kohr and Ingrid W. Caras},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 2},
  pages={1250-7}
}
The COOH terminus of decay accelerating factor (DAF) contains a signal that directs attachment of a glycophosphatidylinositol (GPI) membrane anchor in a process involving proteolytic removal of 17-31 COOH-terminal residues. Previous work suggested that two elements are required for anchor addition, a COOH-terminal hydrophobic domain (the GPI signal) and an element located NH2-terminal to it, postulated to be the cleavage/attachment site. Using [3H]ethanolamine (a component of the anchor) to tag… CONTINUE READING