Glycopeptide Site Heterogeneity and Structural Diversity Determined by Combined Lectin Affinity Chromatography/IMS/CID/MS Techniques

@article{Zhu2015GlycopeptideSH,
  title={Glycopeptide Site Heterogeneity and Structural Diversity Determined by Combined Lectin Affinity Chromatography/IMS/CID/MS Techniques},
  author={Feifei Zhu and Jonathan C. Trinidad and David E. Clemmer},
  journal={Journal of The American Society for Mass Spectrometry},
  year={2015},
  volume={26},
  pages={1092-1102}
}
AbstractGlycopeptides from a tryptic digest of chicken ovomucoid were enriched using a simplified lectin affinity chromatography (LAC) platform, and characterized by high-resolution mass spectrometry (MS) as well as ion mobility spectrometry (IMS)-MS. The LAC platform effectively enriched the glycoproteome, from which a total of 117 glycopeptides containing 27 glycan forms were identified for this protein. IMS-MS analysis revealed a high degree of glycopeptide site heterogeneity. Comparison of… CONTINUE READING
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