Glycomics: an integrated systems approach to structure-function relationships of glycans

  title={Glycomics: an integrated systems approach to structure-function relationships of glycans},
  author={Rahul Raman and S. Raguram and Ganesh Venkataraman and James C. Paulson and Ram Sasisekharan},
  journal={Nature Methods},
In comparison with genomics and proteomics, the advancement of glycomics has faced unique challenges in the pursuit of developing analytical and biochemical tools and biological readouts to investigate glycan structure-function relationships. Glycans are more diverse in terms of chemical structure and information density than are DNA and proteins. This diversity arises from glycans' complex nontemplate-based biosynthesis, which involves several enzymes and isoforms of these enzymes… 
Harnessing glycomics technologies: Integrating structure with function for glycan characterization
Focusing on acidic glycans, key glycomics technologies for characterizing their structural attributes, including linkage, modifications, and topology, as well as for elucidating their role in biological processes are described.
Informatics for Glycans
This review focuses on areas of technologies for sequencing glycans and the importance of developing a bioinformatics platform to integrate the diverse data sets generated using the different technologies to allow a systems approach to glycan structure–function relationships.
Glycomics: an overview of the complex glycocode.
Current progress in glycomics is focused on, including new mass spectrometry-based high-throughput techniques for glycan purification and annotation, the recent work on enhancing microarray methodologies to decipher the glycome, and the scope of glycans as disease markers.
Systems glycobiology: biochemical reaction networks regulating glycan structure and function.
The current state of the art in the application of computational modeling in the field of Glycobiology is summarized with an overview of experimental and online resources that can be used to construct glycosylation reaction networks.
Glycan array data management at Consortium for Functional Glycomics.
This manuscript outlines methodologies for capturing data from glycan array experiments and online tools to access and visualize glycan arrays data implemented at the Consortium for Functional Glycomics.
Glycoproteomics in Health and Disease
Functional glycomics and glycoproteomics are emerging as a central field in systems biology and will continue to be a key focus in discerning health and disease.
Glycomics approach to structure-function relationships of glycosaminoglycans.
This review highlights the important aspects of GAGs and summarizes these aspects in the context of taking a glycomics approach that integrates the different technologies to define structure-function relationships of G AGs.
Glycomic and glycoproteomic analysis of glycoproteins—a tutorial
This tutorial is aimed at researchers from the fields of molecular biology and biochemistry that have discovered that glycoproteins are important in their biological research and are looking for the tools to elucidate their structure.


A genetic approach to Mammalian glycan function.
The study of glycans has entered an era of renaissance that coincides with the acquisition of complete genome sequences for multiple organisms and an increased focus upon how posttranslational modifications to protein contribute to the complexity of events mediating normal and disease physiology.
Lectin-based structural glycomics: Glycoproteomics and glycan profiling
This chapter describes the development of high-throughput and robust machines, which should greatly contribute to the structural analysis of complex glycans, and 2 major research items, i.e., glycoproteomics, which enables comprehensive analysis of Glycoproteins and “glycan profiling” by means of lectins, are described.
Essentials of Glycobiology
General principles - historical background and overview saccharide structure and nomenclature evolution of glycan diversity protein-glycan Interactions exploring the biological roles of glycans
An analytical and structural database provides a strategy for sequencing O-glycans from microgram quantities of glycoproteins.
A sensitive, rapid, quantitative strategy has been developed for O-glycan analysis that provides a basis for the analysis of subpicomole quantities of O- Glycans from normal levels of natural glycoproteins.
Role of glycosylation in development.
A review of recent studies analyzing function of a variety of glycoconjugates, focusing on lessons learned from human disease and genetic studies in mice, Drosophila melanogaster, and Caenorhabditis elegans, suggests that O-fucose, O-mannose, N-glycans, mucin-type O-gly cans and proteoglycans are likely to play important roles in developmental processes.
Exploring the glycan repertoire of genetically modified mice by isolation and profiling of the major glycan classes and nano-NMR analysis of glycan mixtures.
It is found that nano-NMR spectroscopy of whole mixtures of N- and O-glycans can complement HPLC profiling methods for elucidating structural details, and recovery of each major class of glycans is as good or better than with conventional approaches for isolating individual classes.
GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources
GlycoSuiteDB is a relational database that curates information from the scientific literature on glyco-protein derived glycan structures, their biological sources, the references in which the glycan
Glycans in cancer and inflammation — potential for therapeutics and diagnostics
This review highlights changes in glycosylation associated with cancer and chronic inflammation and new therapeutic and diagnostic strategies that are based on the underlying glycobiology.
Glycoprotein Structure Determination by Mass Spectrometry
The power of ultrahigh-sensitivity mass spectrometric strategies for defining the primary structures of highly complex mixtures of glycoprotein glycoforms is set to revolutionize structural glycobiology in the coming postgenomic era.
Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling.
This chapter describes the main issues related to the establishment of a custom glycogenes array, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis.