Glycomics: an integrated systems approach to structure-function relationships of glycans

@article{Raman2005GlycomicsAI,
  title={Glycomics: an integrated systems approach to structure-function relationships of glycans},
  author={R. Raman and S. Raguram and G. Venkataraman and J. Paulson and R. Sasisekharan},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={817-824}
}
In comparison with genomics and proteomics, the advancement of glycomics has faced unique challenges in the pursuit of developing analytical and biochemical tools and biological readouts to investigate glycan structure-function relationships. Glycans are more diverse in terms of chemical structure and information density than are DNA and proteins. This diversity arises from glycans' complex nontemplate-based biosynthesis, which involves several enzymes and isoforms of these enzymes… Expand

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References

SHOWING 1-10 OF 82 REFERENCES
A genetic approach to Mammalian glycan function.
TLDR
The study of glycans has entered an era of renaissance that coincides with the acquisition of complete genome sequences for multiple organisms and an increased focus upon how posttranslational modifications to protein contribute to the complexity of events mediating normal and disease physiology. Expand
Bioinformatics for glycomics: Status, methods, requirements and perspectives
TLDR
The aim is to create a cell-by-cell catalogue of glycosyltransferase expression and detected glycan structures to accept that unrestricted dissemination of scientific data accelerates scientific findings and initiates a number of new initiatives to explore the data. Expand
Lectin-based structural glycomics: Glycoproteomics and glycan profiling
TLDR
This chapter describes the development of high-throughput and robust machines, which should greatly contribute to the structural analysis of complex glycans, and 2 major research items, i.e., glycoproteomics, which enables comprehensive analysis of Glycoproteins and “glycan profiling” by means of lectins, are described. Expand
Essentials of Glycobiology
General principles - historical background and overview saccharide structure and nomenclature evolution of glycan diversity protein-glycan Interactions exploring the biological roles of glycansExpand
Glycomics: a pathway to a class of new and improved therapeutics
TLDR
Recent advances that promise to increase the ability to use complex glycans as therapeutics are outlined and opportunities for the development of further structure–function relationships for these complex molecules are discussed. Expand
An analytical and structural database provides a strategy for sequencing O-glycans from microgram quantities of glycoproteins.
TLDR
A sensitive, rapid, quantitative strategy has been developed for O-glycan analysis that provides a basis for the analysis of subpicomole quantities of O- Glycans from normal levels of natural glycoproteins. Expand
Role of glycosylation in development.
TLDR
A review of recent studies analyzing function of a variety of glycoconjugates, focusing on lessons learned from human disease and genetic studies in mice, Drosophila melanogaster, and Caenorhabditis elegans, suggests that O-fucose, O-mannose, N-glycans, mucin-type O-gly cans and proteoglycans are likely to play important roles in developmental processes. Expand
Exploring the glycan repertoire of genetically modified mice by isolation and profiling of the major glycan classes and nano-NMR analysis of glycan mixtures.
TLDR
It is found that nano-NMR spectroscopy of whole mixtures of N- and O-glycans can complement HPLC profiling methods for elucidating structural details, and recovery of each major class of glycans is as good or better than with conventional approaches for isolating individual classes. Expand
Structural insights into biological roles of protein-glycosaminoglycan interactions.
TLDR
This review provides structural insights into the oligosaccharide-protein interactions and discusses some key and challenging aspects of understanding GAG structure-function relationships. Expand
GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources
GlycoSuiteDB is a relational database that curates information from the scientific literature on glyco-protein derived glycan structures, their biological sources, the references in which the glycanExpand
...
1
2
3
4
5
...