Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius.

  title={Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius.},
  author={R Sk{\'o}rko and Jerzy Osipiuk and K. O. Stetter},
  journal={Journal of bacteriology},
  volume={171 9},
Glycogen-bound polyphosphate kinase has been isolated from a crude extract of Sulfolobus acidocaldarius by isopycnic centrifugation in CsCl. Divalent cations (Mn2+ greater than Mg2+) stimulated the reaction. The enzyme does not require the presence of histones for its activity; it is inhibited strongly by phosphate and slightly by fluoride. The protein from the glycogen complex migrated in a sodium dodecyl sulfate-polyacrylamide gel as a 57-kilodalton protein band; after isoelectric focusing it… CONTINUE READING