Glycochips from polyanionic glycopolymers as tools for detecting Shiga toxins.

@article{Uzawa2007GlycochipsFP,
  title={Glycochips from polyanionic glycopolymers as tools for detecting Shiga toxins.},
  author={Hirotaka Uzawa and Hiroki Ito and Paola Neri and Hiroshi Daikin Industries Mori and Yoshihiro Nishida},
  journal={Chembiochem : a European journal of chemical biology},
  year={2007},
  volume={8 17},
  pages={
          2117-24
        }
}
An alternating layer-by-layer adsorption methodology was applied to the assembly of glycochips by using synthetic polyanionic glycopolymers. Three glycochips carrying globobioside (Gb(2)), beta-lactoside (beta-Lac), or alpha-D-mannoside (alpha-Man) residues were prepared, and used for the detection of Shiga toxins, Stx-1 and Stx-2, by using surface plasmon resonance (SPR). Using this method, we could confirm that both Stx-1 and Stx-2 show binding specificity for the Gb(2) glycochip as well as a… CONTINUE READING
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