Glycine betaine improves survival of fresh bovine spermatozoa.

  title={Glycine betaine improves survival of fresh bovine spermatozoa.},
  author={Bing R Zhang and Matthew Buhr and T Kroetschd and Stanley P. Leibo},
  journal={Reproduction, fertility, and development},
  volume={13 2-3},
Fresh spermatozoa from bulls established as 'good freezers' and 'poor freezers' (consistently > or = 50% or < 20% motile spermatozoa after cryopreservation, respectively) were incubated for 96 h in Tes/Tris-egg yolk or TALP-egg yolk media at 37 degrees, 20 degrees , 5 degrees or 0 degrees C. The TALP extender contained 0, 100 or 200 mM glycine betaine (GB) to test the hypothesis that GB would efficiently maintain spermatozoa function during long-term incubation. The percentage of motile… CONTINUE READING