Glycerol kinase of Trypanosoma brucei. Cloning, molecular characterization and mutagenesis.

@article{Krlov2000GlycerolKO,
  title={Glycerol kinase of Trypanosoma brucei. Cloning, molecular characterization and mutagenesis.},
  author={Ivica Kr{\'a}lov{\'a} and Daniel J. Rigden and Fred R. Opperdoes and Paul A M Michels},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 8},
  pages={2323-33}
}
Trypanosoma brucei contains two tandemly arranged genes for glycerol kinase. The downstream gene was analysed in detail. It contains an ORF for a polypeptide of 512 amino acids. The polypeptide has a calculated molecular mass of 56 363 Da and a pI of 8.6. Comparison of the T. brucei glycerol kinase amino-acid sequence with the glycerol kinase sequences available in databases revealed positional identities of 39.0-50.4%. The T. brucei glycerol kinase gene was overexpressed in Escherichia coli… CONTINUE READING
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