Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro.

@article{Raibekas1996GlycerolinducedDO,
  title={Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro.},
  author={Andrei A Raibekas and Vincent Massey},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 15},
  pages={7546-51}
}
The reconstitutable apoprotein of Crotalus adamanteus L-amino acid oxidase was prepared using hydrophobic interaction chromatography. After reconstitution with flavin adenine dinucleotide, the resulting protein was inactive, with a perturbed conformation of the flavin binding site. Subsequently, a series of cosolvent-dependent compact intermediates was identified. The nearly complete activation of the reconstituted apoprotein and the restoration of its native flavin binding site was achieved in… CONTINUE READING
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