Glyceraldehyde phosphate dehydrogenase oxidation during cardiac ischemia and reperfusion.

  title={Glyceraldehyde phosphate dehydrogenase oxidation during cardiac ischemia and reperfusion.},
  author={Philip Eaton and Neville Bryce Wright and David J. Hearse and Michael J. Shattock},
  journal={Journal of molecular and cellular cardiology},
  volume={34 11},
OBJECTIVES Protein S-glutathiolation is a predicted mechanism by which protein thiol groups are oxidized during the oxidative stress of ischaemia and reperfusion. We measured protein S-thiolation during ischaemia and reperfusion and investigated the effect of this oxidative modification on the function of GAPDH. METHODS Glutathione was biotinylated (biotin-GSH) and used to probe for protein S-glutathiolation in isolated rat hearts using non-reducing Western blots and streptavidin-HRP… CONTINUE READING


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