Glyceraldehyde-3-phosphate dehydrogenase is a reliable internal control in Western blot analysis of leukocyte subpopulations from children.

@article{Yu2011Glyceraldehyde3phosphateDI,
  title={Glyceraldehyde-3-phosphate dehydrogenase is a reliable internal control in Western blot analysis of leukocyte subpopulations from children.},
  author={Hong-Ren Yu and Ho-Chang Kuo and Hsin-Chun Huang and Li-Tung Huang and You-Lin Tain and Chih-Cheng Chen and C P Liang and Jiunn-Ming Sheen and I-Chun Lin and Chi-Chiang Wu and Chi T. Ou and Kuender D. Yang},
  journal={Analytical biochemistry},
  year={2011},
  volume={413 1},
  pages={24-9}
}
To study differences in the development of immunity, leukocytes from cord blood are often compared with those from adult peripheral blood. Western blot analysis is a common method for detecting proteins. In this study, we investigated the reliability of using different housekeeping proteins (β-actin, β-tubulin, and glyceraldehyde-3-phosphate dehydrogenase [GAPDH]) as internal controls for different leukocyte subpopulations from infants, children, and adults. Our results showed that the… CONTINUE READING

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