Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer.

@article{Rvillion2000Glyceraldehyde3phosphateDG,
  title={Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer.},
  author={Françoise R{\'e}villion and Val{\'e}rie Pawlowski and Louis Hornez and Jean Peyrat},
  journal={European journal of cancer},
  year={2000},
  volume={36 8},
  pages={
          1038-42
        }
}
  • Françoise Révillion, Valérie Pawlowski, +1 author Jean Peyrat
  • Published in European journal of cancer 2000
  • Biology, Medicine
  • Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been widely used as a control RNA in Northern blotting and in reverse transcriptase-polymerase chain reaction (RT-PCR) analyses. We investigated the expression of GAPDH in a large series of primary breast cancers and in MCF7 human mammary epithelial breast cancer cells treated with oestradiol. The expression of GAPDH was quantified by a real-time one-step RT-PCR assay, based upon the 5' nuclease activity of Taq polymerase using an Abi Prism… CONTINUE READING

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