Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interacts with apurinic/apyrimidinic sites in DNA.

@article{Kosova2015Glyceraldehyde3phosphateD,
  title={Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interacts with apurinic/apyrimidinic sites in DNA.},
  author={Anastasiya A Kosova and Svetlana N. Khodyreva and Olga D Zakharova},
  journal={Mutation research},
  year={2015},
  volume={779},
  pages={46-57}
}
Apurinic/apyrimidinic (AP) sites are some of the most frequent DNA damages and the key intermediates of base excision repair. Certain proteins can interact with the deoxyribose of the AP site to form a Schiff base, which can be stabilized by NaBH4 treatment. Several types of DNA containing the AP site were used to trap proteins in human cell extracts by this method. In the case of single-stranded AP DNA and AP DNA duplex with both 5' and 3' dangling ends, the major crosslinking product had an… CONTINUE READING

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