Glutathione transferases immobilized on nanoporous alumina: flow system kinetics, screening, and stability.

@article{Kjellander2014GlutathioneTI,
  title={Glutathione transferases immobilized on nanoporous alumina: flow system kinetics, screening, and stability.},
  author={Marcus Kjellander and Aslam M. A. Mazari and Mats Boman and Bengt Mannervik and Gunnar Johansson},
  journal={Analytical biochemistry},
  year={2014},
  volume={446},
  pages={59-63}
}
The previously uncharacterized Drosophila melanogaster Epsilon-class glutathione transferases E6 and E7 were immobilized on nanoporous alumina. The nanoporous anodized alumina membranes were derivatized with 3-aminopropyl-triethoxysilane, and the amino groups were activated with carbonyldiimidazole to allow coupling of the enzymes via ε-amino groups. Kinetic analyses of the immobilized enzymes were carried out in a circulating flow system using CDNB (1-chloro-2,4-dinitrobenzene) as substrate… CONTINUE READING