Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa.

@article{Ansari2012GlutathionesupplementedTA,
  title={Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa.},
  author={Muhammad Ansari and Bushra Allah Rakha and Syed Muntazir Andrabi and Nemat Ullah and Razia Iqbal and William Vincent Holt and S Akhter},
  journal={Reproductive biology},
  year={2012},
  volume={12 3},
  pages={271-6}
}
In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (37°C; 50×10(6)spermatozoaml(-1)) with experimental tris-citric acid extender containing 0, 0.5, 1.0, 1… CONTINUE READING