Glutathione stability in whole blood: effects of various deproteinizing acids.

@article{Stempak2001GlutathioneSI,
  title={Glutathione stability in whole blood: effects of various deproteinizing acids.},
  author={Diana Stempak and Shannon Dallas and Julia Klein and Reina Bendayan and Gideon Koren and Sylvain Baruchel},
  journal={Therapeutic drug monitoring},
  year={2001},
  volume={23 5},
  pages={542-9}
}
High-performance liquid chromatography separation of reduced and oxidized glutathione (GSH and GSSG) in biologic samples using electrochemical detection offers the convenience of both simultaneous quantitation and simple sample preparation. Rapid acidification is required to prevent GSH autooxidation, GSH and GSSG degradation, and precipitate proteins that interfere with analysis. Currently, little consistency exists in the literature regarding acid selection or the feasibility of sample… CONTINUE READING