Glutathione reductase from pea leaves: response to abiotic stress and characterization of the peroxisomal isozyme.

@article{RomeroPuertas2006GlutathioneRF,
  title={Glutathione reductase from pea leaves: response to abiotic stress and characterization of the peroxisomal isozyme.},
  author={Mar{\'i}a C Romero-Puertas and Francisco J Corpas and Luisa M Sandalio and Marina Leterrier and Mar{\'i}a Rodr{\'i}guez-Serrano and Lu{\'i}s Alfonso Del R{\'i}o and J M Palma},
  journal={The New phytologist},
  year={2006},
  volume={170 1},
  pages={43-52}
}
The glutathione reductase (GR; EC 1.6.4.2) isozyme present in peroxisomes has been purified for the first time, and its unequivocal localization in these organelles, by immunogold electron microscopy, is reported. The enzyme was purified c. 21-fold with a specific activity of 9523 units mg(-1) protein, and a yield of 44 microg protein kg(-1) leaves was obtained. The subunit size of the peroxisomal GR was 56 kDa and the isoelectric point was 5.4. The enzyme was recognized by a polyclonal… CONTINUE READING