• Corpus ID: 2595649

Glutathione S-transferases in gastric carcinomas and in adjacent normal gastric epithelium: immunohistochemical and biochemical analyses.

  title={Glutathione S-transferases in gastric carcinomas and in adjacent normal gastric epithelium: immunohistochemical and biochemical analyses.},
  author={D Schipper and M J Wagenmans and Wilbert H. M. Peters and Urbain J. G. M. van Haelst and Theo Wobbes and A A Verhofstad and D. J. Theo Wagener},
  journal={Anticancer research},
  volume={16 6B},
Glutathione S-transferases (GSTs) are enzymes involved in the detoxification of xenobiotics and are divided into four subclasses. Alpha, Mu, Pi and Theta. Most human gastrointestinal tumors contain increased amounts of GST Pi. In order to compare data on the expression of GSTs obtained by biochemical as well as immunohistochemical methods, we characterized the presence of GST Alpha and Pi by Western blot analysis and immunohistochemistry in 22 samples of human gastric carcinoma and adjacent non… 

Tables from this paper

Expression of glutathione S-transferase θ class isoenzymes in human colorectal and gastric cancers

In both normal human colonic and gastric mucosa, GSTT1-1 and GSTT2-2 are present at high levels, whereas after malignant degeneration, expression is not influenced or is even downregulated.

Immunohistochemical Localization of Glutathione S‐Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma

Biochemical analyses revealed that Barrett's epithelium contains lower levels of GST enzyme activity as well as some GST isoforms, as compared with squamous epithelia, which may have consequences for the treatment of these diseases and may contribute to an understanding of the development of these esophageal disorders.

Alteration of cytochrome P-450 and glutathione S-transferase activity in normal and malignant human stomach.

Investigation of stomach tumor and tumor-adjacent tissues of patients with gastric adenocarcinoma and unaffected individuals suggests alterations in the activities of cytochrome P450 and GST may in part be associated with an increased risk for gastric cancer.

Expression of the xenobiotic- and reactive oxygen species-detoxifying enzymes, GST-pi, Cu/Zn-SOD, and Mn-SOD in the endocrine cells of colorectal cancer

Not the neuroendocrine differentiation in general, but the presence in the tumors of endocrine cells with activated antioxidant defense and probably metabolically more active might determine a more aggressive type of cancer leading to worse prognosis for patients.

Expression of Glutathione S‐Transfer α, P1–1 and T1–1 in the Human Gastrointestinal Tract

The common expression of GSTα, GSTT1–1 and GSTP1-1 in many cell types along the human gastrointestinal tract suggests an important role in the protection against carcinogens and other xenobiotics.


The role of GST-Pi in liver damage, oxidative stress, carcinogenesis and drug resistance are discussed, and the presence of common genetic polymorphism,hypermethylation in GST- Pi gene and the consequences GST- pi knock out is regarded.

GST-pi expression in BCR-ABL+ and BCR-ABL- cells from CML patients.

Observations during haematopoiesis in BMSC liquid cultures from CML patients who were candidates for transplant GST-pi was expressed in presumably malignant cells during different stages of cellular maturation are confirmed and suggest that GST- pi expression might be used for the evaluation of the minimal residual disease in CML Patients.

Prevention of N-methyl-N ′-nitro-N-nitrosoguanidine and saturated sodium chloride-induced gastric carcinogenesis in Wistar rats by lycopene

The data suggest that lycopene may exert its inhibitory effects by modulating the oxidant and antioxidant status in the gastric mucosa of tumour-bearing animals.



Expression of glutathione S-transferases in normal gastric mucosa and in gastric tumors.

Both total GST enzyme activity as well as the absolute amounts of GST-pi protein were significantly higher in the tumors, as compared to its matched normal mucosa, suggesting a role for GST-PI in the mechanism of anti-cancer drug resistance.

Glutathione S-transferase in human esophageal carcinoma.

The marked expression of GST-PI by non-pathologic mucosa obtained from the patients with esophageal carcinoma suggests that GST-pi may be a marker of increased carcinogen exposure, but not necessarily a marker for developed human esophagesia cancer.

Glutathione S-transferase isoenzymes in human renal carcinoma demonstrated by immunohistochemistry.

Using specific polyclonal rabbit antisera, it is demonstrated by immunohistochemistry that all 12 renal carcinomas studied contained GST pi and most tumours also contained GST alpha, GST mu and microsomal GST isoenzymes but their distribution was heterogeneous and sometimes very focal.

Overexpression of P-glycoprotein and glutathione S-transferase-pi in resistant non-small cell lung carcinomas of smokers.

There exists no relationship between resistance and smoking for adenocarcinomas of the lung and significant correlations also exist between resistance in vitro and expression of P-170 or expression of GST-pi.

Glutathione transferases and cancer.

New findings regarding the respective molecular forms involved in carcinogenesis and anticancer drug resistance are reviewed, with particular emphasis on Pi class forms in preneoplastic tissues.

A pilot study of pi-class glutathione S-transferase expression in breast cancer: correlation with estrogen receptor expression and prognosis in node-negative breast cancer.

  • L. GilbertL. Elwood J. Moscow
  • Medicine, Biology
    Journal of clinical oncology : official journal of the American Society of Clinical Oncology
  • 1993
GST pi expression is inversely related to hormone receptor status in breast cancer, and this pilot study suggests that increased GST pi expression may be an important predictor of early recurrence and death in node-negative breast cancer patients that merits additional investigation.

Contribution of glutathione and glutathione‐dependent enzymes in the reversal of adriamycin resistance in colon carcinoma cell lines

Observations suggest that glutathione‐mediated detoxification of Adriamycin may play a role in the resistance of this sub‐line, and suggest a role for drug‐resistance‐modulating agents in the treatment of colon carcinoma.

Glutathione s‐transferase detoxication enzymes in cervical neoplasia

Altered expression of the glutathione S‐transferases (GSTs) has been implicated in the progression to tumour after exposure to carcinogens, and GST Pi has been suggested as a possible marker of