Glutathione-S-transferase: role in buffalo (Bubalus bubalis) sperm capacitation and cryopreservation.

  title={Glutathione-S-transferase: role in buffalo (Bubalus bubalis) sperm capacitation and cryopreservation.},
  author={Raj Kumar and Vinai Kumar Singh and Suresh Kumar Atreja},
  volume={81 4},
In this study, glutathione-S-transferase Mu3 (GST) has been reported to play an important role in sperm capacitation, acrosome reaction, and fertilization. The freshly ejaculated buffalo spermatozoa were in vitro capacitated using heparin (10 μg/mL) or cryopreserved in egg yolk citrate extender. Glutathione-S-transferase was identified and characterized in terms of their isozymic forms, tyrosine phosphorylation, and immunolocalization patterns in cryopreserved buffalo spermatozoa in comparison… CONTINUE READING