Glutaredoxin from calf thymus. Purification to homogeneity.

  title={Glutaredoxin from calf thymus. Purification to homogeneity.},
  author={Margaretha Luthman and Arne Holmgren},
  journal={The Journal of biological chemistry},
  volume={257 12},
The protein glutaredoxin, required for GSH-dependent ribonucleotide reduction, has been purified to homogeneity from calf thymus. The preparative method consisted of ammonium sulfate precipitation and three chromatography steps on DEAE-cellulose, Sephadex G-50, and CM-Sepharose. Calf thymus glutaredoxin was assayed on the basis of its inherent GSH-disulfide transhydrogenase activity. Glutaredoxin, purified 3000-fold was demonstrated to be homogeneous by polyacrylamide gel electrophoresis and… CONTINUE READING


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