Glutaredoxin from calf thymus. Purification to homogeneity.

@article{Luthman1982GlutaredoxinFC,
  title={Glutaredoxin from calf thymus. Purification to homogeneity.},
  author={Margaretha Luthman and Arne Holmgren},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 12},
  pages={6686-90}
}
The protein glutaredoxin, required for GSH-dependent ribonucleotide reduction, has been purified to homogeneity from calf thymus. The preparative method consisted of ammonium sulfate precipitation and three chromatography steps on DEAE-cellulose, Sephadex G-50, and CM-Sepharose. Calf thymus glutaredoxin was assayed on the basis of its inherent GSH-disulfide transhydrogenase activity. Glutaredoxin, purified 3000-fold was demonstrated to be homogeneous by polyacrylamide gel electrophoresis and… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 21 extracted citations

Vertebrate-specific glutaredoxin is essential for brain development.

Proceedings of the National Academy of Sciences of the United States of America • 2011

Similar Papers

Loading similar papers…