Glutamine synthetase in experimental meningitis: increased ratio of the subunits 3 and 2 may indicate enhanced activity.

@article{Smirnov2000GlutamineSI,
  title={Glutamine synthetase in experimental meningitis: increased ratio of the subunits 3 and 2 may indicate enhanced activity.},
  author={Alexey V. Smirnov and Hayrettin Tumani and S Henne and S Barchfeld and U Olgem{\"o}ller and Jens Wiltfang and Peter de Lange and Michael M{\"a}der and Roland Nau},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2000},
  volume={292 1-2},
  pages={1-12}
}
Glutamine synthetase (GS) activity is higher in the neocortex but not in the hippocampal formation of rabbit brain during Streptococcus pneumoniae meningitis compared to the respective brain region of uninfected control animals. One-dimensional polyacrylamide gel electrophoresis (1D-SDS-PAGE) revealed an apparent molecular mass (M(r)) of 44000 Dalton (Da) for GS from rabbit brain. After two-dimensional gel electrophoresis (2D-PAGE), followed by Coomassie-blue staining, GS separated into three… CONTINUE READING