Glutamic acid decarboxylase cDNA: nucleotide sequence encoding an enzymatically active fusion protein.

@article{Kobayashi1987GlutamicAD,
  title={Glutamic acid decarboxylase cDNA: nucleotide sequence encoding an enzymatically active fusion protein.},
  author={Yukari Kobayashi and Daniel L Kaufman and Allan J. Tobin},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1987},
  volume={7 9},
  pages={2768-72}
}
Glutamic acid decarboxylase (GAD;E.C. 4.1.1.15) catalyzes the production of GABA, the major inhibitory neurotransmitter in the mammalian brain. We recently isolated a lambda gt-11 recombinant, lambda-GAD, that contains the cDNA for GAD from feline brain (Kaufman et al., 1986). Interestingly, the beta-galactosidase-GAD fusion protein encoded by lambda GAD is enzymatically active, catalyzing the conversion of glutamate to CO2 and GABA. Here we report the nucleotide sequence of feline GAD cDNA. It… CONTINUE READING
51 Citations
32 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 51 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 32 references

In situ detection of GAD mRNA in mouse brain

  • A. J. Tobin
  • 1986
Highly Influential
4 Excerpts

Monoclonal antibodies to glutamic acid decarboxylase

  • D. I. Gottlieb, Y.-C. Chang, J. E. Schwab
  • Proc. Natl. Acad. Sci. USA
  • 1986
Highly Influential
8 Excerpts

NGF induction of glutamate decarboxylase (GAD) mRNA in pheochromocytoma cell &es

  • N.J.K. Tillakaratne, S. L. Huttner, A. J. Tobin
  • Sot. Neurosci: Abstr
  • 1985
Highly Influential
2 Excerpts

Purification and characterization of glutamate decarboxylase from mouse brain

  • Wu, J.-Y, T. Matsuda, E. Roberts
  • J. Biol
  • 1973
Highly Influential
5 Excerpts

Similar Papers

Loading similar papers…