Glutamic acid decarboxylase autoantibody assay using 125I-labelled recombinant GAD65 produced in yeast.

@article{Powell1996GlutamicAD,
  title={Glutamic acid decarboxylase autoantibody assay using 125I-labelled recombinant GAD65 produced in yeast.},
  author={Michael Powell and Leah Prentice and Takayuki Asawa and Ryoji Kato and Joanna Sawicka and Hiroaki Tanaka and Volker Petersen and A Munkley and Sherwin Morgan and Bernard Rees Smith and Jadwiga Furmaniak},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1996},
  volume={256 2},
  pages={175-88}
}
We describe a new method for measuring autoantibodies (Ab) to the 65 kDa isoform of glutamic acid carboxylase (GAD65). In particular, GAD65 without the hydrophobic N-terminal region has been produced in yeast, purified, labelled with 125I and reacted with GAD65 Ab. Antibody bound 125I-GAD65 is then precipitated by the addition of solid phase protein A. With the assay, GAD65 Ab were detected in 59 of 71 (83%) islet cell antibody (ICA) positive IDDM patients and in 8 of 23 (35%) ICA negative IDDM… CONTINUE READING
26 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 26 extracted citations

Similar Papers

Loading similar papers…