Glutamate synthase from rice leaves.

  title={Glutamate synthase from rice leaves.},
  author={Akira Suzuki and Pierre Gadal},
  journal={Plant physiology},
  volume={69 4},
Ferredoxin-dependent glutamate synthase (EC from rice leaves (Oryza sativa L. cv Delta) was purified 206-fold with a final specific activity of 35.9 mumoles glutamate formed per min per milligram protein by a procedure including ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephacryl S-300 gel filtration, and ferredoxin-Sepharose affinity chromatography. The purified enzyme yielded a single protein band on polyacrylamide gel electrophoresis. Molecular weight of the… CONTINUE READING