Glutamate overcomes the salt inhibition of DNA polymerase III holoenzyme.

@article{Griep1989GlutamateOT,
  title={Glutamate overcomes the salt inhibition of DNA polymerase III holoenzyme.},
  author={Mark A. Griep and Charles S McHenry},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 19},
  pages={11294-301}
}
Even though Escherichia coli can grow in media containing up to 1 M NaCl, one-fifth that amount of NaCl will completely inhibit the in vitro activity of DNA polymerase III holoenzyme. It has been established that the major intracellular ionic osmolytes are potassium and glutamate (Richey, B., Cayley, D. S., Mossing, M. C., Kolka, C., Anderson, C. F., Farrar, T. C., and Record, M. T., Jr. (1987) J. Biol. Chem. 262, 7157-7164). We have found that holoenzyme catalyzes replication efficiently in… CONTINUE READING

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