Glutamate levels in cerebrospinal fluid in amyotrophic lateral sclerosis: a reappraisal using a new HPLC method with coulometric detection in a large cohort of patients

@article{SpreuxVaroquaux2002GlutamateLI,
  title={Glutamate levels in cerebrospinal fluid in amyotrophic lateral sclerosis: a reappraisal using a new HPLC method with coulometric detection in a large cohort of patients},
  author={Odile Spreux‐Varoquaux and Gilbert Bensimon and Lucette Lacomblez and François Salachas and Pierre Pradat and Nadine Le Forestier and Abdellatif Marouan and Michel Dib and Vincent Meininger},
  journal={Journal of the Neurological Sciences},
  year={2002},
  volume={193},
  pages={73-78}
}
Plasma glutamate and glycine levels in patients with amyotrophic lateral sclerosis.
TLDR
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TLDR
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Markers of Excitotoxicity in Patients: Tool for Diagnosis or Therapy?
TLDR
A number of systemic factors, such as genetics, oxidative stress and inflammatory mediators, may be responsible for the observed glutamate uptake impairment in neurological patients.
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TLDR
The CSF biomarkers may give insight into ALS pathophysiology and may be useful for early, presymptomatic diagnosis, therapeutic monitoring and the development of new therapeutic strategies.
Biomarkers in Amyotrophic Lateral Sclerosis
TLDR
An overview of recently described biologic and radiologic markers of the disease is provided, namely the presence of inclusions staining positively for ubiquitin and TAR DNA-binding protein (TARDBP) in degenerating motor neurons.
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TLDR
The findings do not lend support to the hypothesis that the sporadic form of ALS results from overexcitation of motor neurons by excitatory amino acids, and concentrations of glutamate, aspartate, and glycine were normal in the CSF of all 17 patients examined.
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TLDR
Oral glutamate loading resulted in significantly greater elevations in the plasma glutamate and aspartate levels in the ALS patients than in the controls, suggesting a systemic defect in the metabolism of this amino acid may underlie primary ALS.
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The identification of a subgroup of MND patients with high CSF glutamate levels may be important in evaluating the clinical response to antiglutamate therapeutic agents.
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The data from serum and CSF indicate that in ALS an imbalance between excitatory and inhibitory amino acids might be present in the brain, which may be induced in different ways in particular ALS patients.
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TLDR
The results do not support an abnormal profile of excitatory AA concentrations in ALS, and the heterogeneous changes observed, mainly concerning LNAAs, may be explained by a blood‐CSF barrier disturbance in the disease.
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TLDR
It is concluded that excitatory amino acid metabolism is altered in patients with ALS and these changes may play a role in motor neuron loss in ALS.
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TLDR
Assessment of reports of abnormal levels of free amino acids in patients with amyotrophic lateral sclerosis found that the severity of ALS correlated inversely with acidic AA (glutamate and aspartate) and O‐phosphoserine in cerebrospinal fluid.
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TLDR
It is suggested that the reduced levels of glutamate and aspartate as well as the elevated GDH activity in the spinal cord of ALS patients may reflect an overactivity of the neurons releasing these potentially excitotoxic amino acids and thus may be causally related to the spinal neuro-degenerative changes characteristic of ALS.
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TLDR
Five amino acids were significantly elevated in the cerebrospinal fluid of the motor neuron disease patients compared to the controls and these were isoleucine, glycine, alanine, phenylalanine, and threonine, which were increased by 60, 58, 38, 26, and 25% respectively.
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TLDR
A method of inactivating CSF enzymes that yields stable glutamate levels under different storage conditions is suggested, which may explain the discrepancies observed with different methods of CSF processing and analysis.
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