Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation.

Using the fluorescent dye 2',7'-dichlorodihydrofluorescein (DCF-H2) we investigated the role of glutamate in the production of reactive oxygen species (ROS) in cultured neurons from fetal rat forebrain. The addition of an excitotoxic concentration of glutamate (100 microM) produced a generalized decrease in cellular DCF fluorescence accompanied by local… CONTINUE READING