Glutamate-115 renders specificity of human 11beta-hydroxysteroid dehydrogenase type 2 for the cofactor NAD+.

@article{Arnold2003Glutamate115RS,
  title={Glutamate-115 renders specificity of human 11beta-hydroxysteroid dehydrogenase type 2 for the cofactor NAD+.},
  author={Peter Arnold and Steven Tam and Lisa Yan and Michael E. Baker and Felix J. Frey and Alex Odermatt},
  journal={Molecular and cellular endocrinology},
  year={2003},
  volume={201 1-2},
  pages={177-87}
}
The renal 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 2 catalyzes the NAD(+)-dependent oxidation of the C11-alcohol on cortisol and corticosterone to yield inactive 11-ketosteroids. The lack of purified active enzyme complicates structure-function analyses of 11beta-HSD2. Here, we constructed a 3D-structural model of 11beta-HSD2, based on known 3D-structures of other short-chain dehydrogenases/reductases (SDR), and functionally analyzed 11beta-HSD2 mutants predicted to be involved in… CONTINUE READING

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