Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester

@article{Sunner2015GlucuronoylES,
  title={Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester},
  author={Hampus Sunner and Maria-Despoina Charavgi and Lisbeth Olsson and Evangelos Topakas and Paul Christakopoulos},
  journal={Molecules},
  year={2015},
  volume={20},
  pages={17807 - 17817}
}
Research on glucuronoyl esterases (GEs) has been hampered by the lack of enzyme assays based on easily obtainable substrates. While benzyl d-glucuronic acid ester (BnGlcA) is a commercially available substrate that can be used for GE assays, several considerations regarding substrate instability, limited solubility and low apparent affinities should be made. In this work we discuss the factors that are important when using BnGlcA for assaying GE activity and show how these can be applied when… 
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Microbial Glucuronoyl Esterases: 10 Years after Discovery
  • P. Biely
  • Chemistry, Medicine
    Applied and Environmental Microbiology
  • 2016
TLDR
Current knowledge of GEs is a brief summary of current knowledge of these relatively recent and unexplored esterases, which make GEs interesting candidates for biotechnological applications in plant biomass processing and genetic modification of plants.
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Molecular modeling and site-directed mutagenesis studies of AfGE demonstrated that Lys209 plays an important role in the preference for the substrates containing 4-O-methyl group in the glucopyranose ring.
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