Glucosylation of Rho GTPases by Clostridium difficile toxin A triggers apoptosis in intestinal epithelial cells.

@article{Gerhard2008GlucosylationOR,
  title={Glucosylation of Rho GTPases by Clostridium difficile toxin A triggers apoptosis in intestinal epithelial cells.},
  author={Ralf Gerhard and Stefanie Nottrott and Janett Schoentaube and Helma Tatge and Alexandra Olling and Ingo Just},
  journal={Journal of medical microbiology},
  year={2008},
  volume={57 Pt 6},
  pages={
          765-70
        }
}
The intestinal epithelial cell line HT-29 was used to study the apoptotic effect of Clostridium difficile toxin A (TcdA). TcdA is a 300 kDa single-chain protein, which glucosylates and thereby inactivates small GTPases of the Rho family (Rho, Rac and Cdc42). The effect of TcdA-catalysed glucosylation of the Rho GTPases is well known: reorganization of the actin cytoskeleton with accompanying morphological changes in cells, leading to complete rounding of cells and destruction of the intestinal… 

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