Glucosylated Hydroxymethyluracil, DNA Base J, Prevents Transcriptional Readthrough in Leishmania

  title={Glucosylated Hydroxymethyluracil, DNA Base J, Prevents Transcriptional Readthrough in Leishmania},
  author={Henri G.A.M. van Luenen and Carol Farris and Sabrina Z. Jan and Paul-Andr{\'e} Genest and Pankaj Tripathi and Arno Velds and Ron M. Kerkhoven and Marja Nieuwland and Andrew K Haydock and Gowthaman Ramasamy and Saara Vainio and Tatjana Heidebrecht and Anastassis Perrakis and Ludo Pagie and Bas van Steensel and Peter J. Myler and Piet Borst},
Some Ts in nuclear DNA of trypanosomes and Leishmania are hydroxylated and glucosylated to yield base J (β-D-glucosyl-hydroxymethyluracil). In Leishmania, about 99% of J is located in telomeric repeats. We show here that most of the remaining J is located at chromosome-internal RNA polymerase II termination sites. This internal J and telomeric J can be reduced by a knockout of J-binding protein 2 (JBP2), an enzyme involved in the first step of J biosynthesis. J levels are further reduced by… CONTINUE READING
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Localization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei

  • F. van Leeuwen, E. R. Wijsman, R. Kieft, G. A. van der Marel, J. H. van Boom, P. Borst
  • Genes Dev
  • 1997
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