Glucose transport and transporters in muscle giant vesicles: differential effects of insulin and contractions.


Collagenase treatment of skeletal muscle results in the formation of large spheres of membranes (3-30 microns diam). A procedure is described for purification and concentration of these giant membrane vesicles prepared from rat muscle. Morphological observations, marker enzyme analysis, and immunoblotting demonstrate that the vesicles are of plasma membrane… (More)


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