Glucose induces glucose 6-phosphatase hydrolytic subunit gene transcription in an insulinoma cell line (INS-1).

@article{Schmoll1999GlucoseIG,
  title={Glucose induces glucose 6-phosphatase hydrolytic subunit gene transcription in an insulinoma cell line (INS-1).},
  author={Dieter Schmoll and Sandra L. Watkins and Christina Wasner and R. {\'A}lvarez-Sala Walther and A. R. Burchell},
  journal={FEBS letters},
  year={1999},
  volume={443 1},
  pages={53-6}
}
Primer extension analysis and RNase protection assays revealed the identity of glucose 6-phosphatase gene transcripts in both the insulinoma cell line INS-1 and hepatic cells. In transient transfection assays of INS-1 cells, using constructs between the human glucose 6-phosphatase gene promoter and a luciferase reporter gene, the reporter gene activity was induced by dexamethasone and dibutyryl cAMP. Furthermore, the promoter was regulated by the glucose concentration in the medium. This effect… CONTINUE READING