Glucose Dehydrogenase for the Regeneration of NADPH and NADH

@inproceedings{Weckbecker2005GlucoseDF,
  title={Glucose Dehydrogenase for the Regeneration of NADPH and NADH},
  author={Andrea Weckbecker and Werner Hummel},
  year={2005}
}
Glucose dehydrogenases (GDHs) occur in several organisms such as Bacillus megaterium and Bacillus subtilis. They accept both NAD+ and NADP+ as cofactor and can be used for the regeneration of NADH and NADPH. In order to demonstrate their applicability we coupled an NADP+-dependent, (R)-specific alcohol dehydrogenase (ADH) from Lactobacillus kefir with the glucose dehydrogenase from B. subtilis. The ADH reduces prochiral ketones stereoselectively to chiral alcohols. The reduction requires NADPH… CONTINUE READING

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