Glucosamine-induced Insulin Resistance in 3T3-L1 Adipocytes Is Caused by Depletion of Intracellular ATP*

@article{Hresko1998GlucosamineinducedIR,
  title={Glucosamine-induced Insulin Resistance in 3T3-L1 Adipocytes Is Caused by Depletion of Intracellular ATP*},
  author={Richard C. Hresko and Harry Heimberg and Michael M. Chi and Mike M. Mueckler},
  journal={The Journal of Biological Chemistry},
  year={1998},
  volume={273},
  pages={20658 - 20668}
}
Glucosamine, which enters the hexosamine pathway downstream of the rate-limiting step, has been routinely used to mimic the insulin resistance caused by high glucose and insulin. We investigated the effect of glucosamine on insulin-stimulated glucose transport in 3T3-L1 adipocytes. The Δ-insulin (insulin-stimulated minus basal) value for 2-deoxyglucose uptake was dramatically inhibited with increasing concentrations of glucosamine with an ED50 of 1.95 mm. Subcellular fractionation experiments… Expand

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