Glucosamine enhances platelet-derived growth factor-induced DNA synthesis via phosphatidylinositol 3-kinase pathway in rat aortic smooth muscle cells.

  title={Glucosamine enhances platelet-derived growth factor-induced DNA synthesis via phosphatidylinositol 3-kinase pathway in rat aortic smooth muscle cells.},
  author={Akira Sato and Toshiyasu Sasaoka and K. Yamazaki and Norio Nakamura and Rie Temaru and Manabu Ishiki and Michiyo Takata and Mika Kishida and Tsutomu Wada and Hajime Ishihara and Isao Usui and Masaharu Urakaze and M. Kobayashi},
  volume={157 2},
Vascular smooth muscle cells play a key role in the development of atherosclerosis. Culture of vascular smooth muscle A10 cells with high glucose for 4 weeks enhanced platelet-derived growth factor (PDGF)-induced BrdU incorporation. Since a long period of high glucose incubation was required for the effect, and it was inhibited by co-incubation with azaserine, the role of hexosamine biosynthesis in the development of atherosclerosis in diabetes was studied in A10 cells. Addition of glucosamine… Expand
High glucose potentiates mitogenic responses of cultured ovine coronary smooth muscle cells to platelet derived growth factor and transforming growth factor-beta1.
High glucose, per se, only very weakly stimulates smooth muscle cell growth but it interacts positively to potentiate the responses to the vascular derived growth factors PDGF and TGF-beta1. Expand
Glucose-Potentiated Chemotaxis in Human Vascular Smooth Muscle Is Dependent on Cross-Talk Between the PI3K and MAPK Signaling Pathways
Glucose sensitizes VSMC to serum, inducing chemotaxis via pathways involving p110&bgr;-PI3K, Akt, mTOR, and ERK1/2 MAPK, indicating a role for the Ras family of GTPases (MAPK pathway) under these conditions. Expand
Dual role of SRC homology domain 2-containing inositol phosphatase 2 in the regulation of platelet-derived growth factor and insulin-like growth factor I signaling in rat vascular smooth muscle cells.
The results suggest that SHIP2 regulates PDGF- and IGF-I-mediated signaling downstream of PI3-kinase, leading to the antiapoptotic effect via 5-phosphatase activity, and that SHip2 regulates the growth factor-induced Ras-MAPK pathway mainly via the SH2 domain. Expand
Anti-proliferative activity of oral anti-hyperglycemic agents on human vascular smooth muscle cells: thiazolidinediones (glitazones) have enhanced activity under high glucose conditions
TZDs but not sulfonylureas nor biguanides (except phenformin at high concentrations) show favorable vascular actions assessed as inhibition of vSMC proliferation, and the activity of rosiglitazone and piog litazone is enhanced under high glucose conditions. Expand
Glucosamine Sodium Sulfate Can Penetrate Skin and May Affect Glucose Metabolism in Rats
Oral administration of GlcNs does not affect body weight, but may influence glucose metabolism in rats, important for research concerning diabetes and its complications in patients with arthritis. Expand
GLUT2 is a high affinity glucosamine transporter
Glucosamine transport by GLUT2 was confirmed in mammalian cells and, using hepatocytes from control orGLUT2‐null mice, HgCl2‐inhibitable glucosamine uptake by liver was shown to be exclusively through GLUT1 and GLUT4. Expand
High performance liquid chromatography for the determination of glucosamine sulfate in human plasma after derivatization with 9-fluorenylmethyl chloroformate.
A simple, rapid, sensitive, and reliable method based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by reverse-phase HPLC-FLD, which was shown to be selective and sensitive for glucosamine sulfate. Expand


Enhanced Expression of Platelet-Derived Growth Factor-β Receptor by High Glucose Involvement of Platelet-Derived Growth Factor in Diabetic Angiopathy
The results suggest that stimulation of the PDGF system is significantly involved in the development not only of diabetic atherosclerosis but also of microangiopathy. Expand
Platelet-derived growth factor stimulates growth factor receptor binding protein-2 association with Shc in vascular smooth muscle cells.
It is concluded that Shc serves as a primary docking protein for GRB2 in smooth muscle cells and is critical for proliferation in response to PDGF. Expand
High glucose-induced transforming growth factor beta1 production is mediated by the hexosamine pathway in porcine glomerular mesangial cells.
The study indicates that the flux of glucose metabolism through the GFAT catalyzed hexosamine biosynthetic pathway is involved in the glucose-induced mesangial production of TGF-beta leading to increased matrix production. Expand
Evidence for involvement of phospholipase C-gamma 2 in signal transduction of platelet-derived growth factor in vascular smooth-muscle cells.
The expression of PLC-gamma 2 was extremely low in the primary VSMC cultures and was induced during further cultivation of the primary cultures, indicating that an acquisition of PDGF-signal-transducing components, including PLC, may be an important step for proliferation of smooth-muscle cells. Expand
Glucose and glucosamine regulate growth factor gene expression in vascular smooth muscle cells.
High glucose concentrations such as are reached in diabetes mellitus can stimulate the transcription of the genes for growth factors in vascular smooth muscle cells, which could contribute to the toxicity of hyperglycemia and the vascular complications of diabetes. Expand
Patterns of tyrosine phosphorylation differ in vascular hypertrophy and hyperplasia.
  • S. Ali, G. Dorn
  • Chemistry, Medicine
  • The American journal of physiology
  • 1994
Results indicate that tyrosine phosphorylation of PLC-gamma 1, GAP, and PI-3-kinase are specific responses for VSMC hyperplasia but not thromboxane-stimulated hypertrophy. Expand
Glucose Metabolism to Glucosamine Is Necessary for Glucose Stimulation of Transforming Growth Factor-α Gene Transcription*
Results indicate that the metabolism of glucose to glucosamine is necessary for the transcriptional stimulation of TGFα expression in vascular smooth muscle cells by glucose, and the level of glycosylation of some intracellular proteins can be modulated in response to physiological changes in the extracellular glucose concentration and the net activity of GFAT. Expand
Platelet-derived growth factor stimulates formation of active p21ras.GTP complex in Swiss mouse 3T3 cells.
The results strongly suggest that p21 is a transducer of the growth signal from the platelet-derived growth factor receptor in Swiss 3T3 cells and that the signal is transmitted through a p21.GTP complex. Expand
Glucose regulation of transforming growth factor-alpha expression is mediated by products of the hexosamine biosynthesis pathway.
Maximal stimulation of TGF alpha-luciferase activity by glucosamine was equivalent in GFA-and control-transfected cells, confirming that the stimulation observed by both agents operated through the same pathway. Expand
Aldose reductase inhibitor prevents hyperproliferation and hypertrophy of cultured rat vascular smooth muscle cells induced by high glucose.
Results indicate that this aldose reductase inhibitor (ARI) possesses an antiproliferative and antihypertrophic action on VSMCs induced by high glucose possibly through protein kinase C suppression. Expand