Glucosamine enhances platelet-derived growth factor-induced DNA synthesis via phosphatidylinositol 3-kinase pathway in rat aortic smooth muscle cells.

@article{Sato2001GlucosamineEP,
  title={Glucosamine enhances platelet-derived growth factor-induced DNA synthesis via phosphatidylinositol 3-kinase pathway in rat aortic smooth muscle cells.},
  author={Akira Sato and Toshiyasu Sasaoka and K. Yamazaki and Norio Nakamura and Rie Temaru and Manabu Ishiki and Michiyo Takata and Mika Kishida and Tsutomu Wada and Hajime Ishihara and Isao Usui and Masaharu Urakaze and M. Kobayashi},
  journal={Atherosclerosis},
  year={2001},
  volume={157 2},
  pages={
          341-52
        }
}
Vascular smooth muscle cells play a key role in the development of atherosclerosis. Culture of vascular smooth muscle A10 cells with high glucose for 4 weeks enhanced platelet-derived growth factor (PDGF)-induced BrdU incorporation. Since a long period of high glucose incubation was required for the effect, and it was inhibited by co-incubation with azaserine, the role of hexosamine biosynthesis in the development of atherosclerosis in diabetes was studied in A10 cells. Addition of glucosamine… Expand
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References

SHOWING 1-10 OF 43 REFERENCES
Enhanced Expression of Platelet-Derived Growth Factor-β Receptor by High Glucose Involvement of Platelet-Derived Growth Factor in Diabetic Angiopathy
TLDR
The results suggest that stimulation of the PDGF system is significantly involved in the development not only of diabetic atherosclerosis but also of microangiopathy. Expand
Platelet-derived growth factor stimulates growth factor receptor binding protein-2 association with Shc in vascular smooth muscle cells.
TLDR
It is concluded that Shc serves as a primary docking protein for GRB2 in smooth muscle cells and is critical for proliferation in response to PDGF. Expand
High glucose-induced transforming growth factor beta1 production is mediated by the hexosamine pathway in porcine glomerular mesangial cells.
TLDR
The study indicates that the flux of glucose metabolism through the GFAT catalyzed hexosamine biosynthetic pathway is involved in the glucose-induced mesangial production of TGF-beta leading to increased matrix production. Expand
Evidence for involvement of phospholipase C-gamma 2 in signal transduction of platelet-derived growth factor in vascular smooth-muscle cells.
TLDR
The expression of PLC-gamma 2 was extremely low in the primary VSMC cultures and was induced during further cultivation of the primary cultures, indicating that an acquisition of PDGF-signal-transducing components, including PLC, may be an important step for proliferation of smooth-muscle cells. Expand
Glucose and glucosamine regulate growth factor gene expression in vascular smooth muscle cells.
TLDR
High glucose concentrations such as are reached in diabetes mellitus can stimulate the transcription of the genes for growth factors in vascular smooth muscle cells, which could contribute to the toxicity of hyperglycemia and the vascular complications of diabetes. Expand
Patterns of tyrosine phosphorylation differ in vascular hypertrophy and hyperplasia.
  • S. Ali, G. Dorn
  • Chemistry, Medicine
  • The American journal of physiology
  • 1994
TLDR
Results indicate that tyrosine phosphorylation of PLC-gamma 1, GAP, and PI-3-kinase are specific responses for VSMC hyperplasia but not thromboxane-stimulated hypertrophy. Expand
Glucose Metabolism to Glucosamine Is Necessary for Glucose Stimulation of Transforming Growth Factor-α Gene Transcription*
TLDR
Results indicate that the metabolism of glucose to glucosamine is necessary for the transcriptional stimulation of TGFα expression in vascular smooth muscle cells by glucose, and the level of glycosylation of some intracellular proteins can be modulated in response to physiological changes in the extracellular glucose concentration and the net activity of GFAT. Expand
Platelet-derived growth factor stimulates formation of active p21ras.GTP complex in Swiss mouse 3T3 cells.
TLDR
The results strongly suggest that p21 is a transducer of the growth signal from the platelet-derived growth factor receptor in Swiss 3T3 cells and that the signal is transmitted through a p21.GTP complex. Expand
Glucose regulation of transforming growth factor-alpha expression is mediated by products of the hexosamine biosynthesis pathway.
TLDR
Maximal stimulation of TGF alpha-luciferase activity by glucosamine was equivalent in GFA-and control-transfected cells, confirming that the stimulation observed by both agents operated through the same pathway. Expand
Aldose reductase inhibitor prevents hyperproliferation and hypertrophy of cultured rat vascular smooth muscle cells induced by high glucose.
TLDR
Results indicate that this aldose reductase inhibitor (ARI) possesses an antiproliferative and antihypertrophic action on VSMCs induced by high glucose possibly through protein kinase C suppression. Expand
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