Glucocorticoids suppress T cell function by up-regulating microRNA-98.

  title={Glucocorticoids suppress T cell function by up-regulating microRNA-98.},
  author={Trevor E. Davis and Katalin Kis-T{\'o}th and Attila Sz{\'a}nt{\'o} and George C Tsokos},
  journal={Arthritis and rheumatism},
  volume={65 7},
OBJECTIVE To identify microRNAs (miRNAs) in human T cells that can explain known antiinflammatory properties of steroids. METHODS Activated human CD4+ T cells from healthy donors were exposed to 1 μM methylprednisolone (MP) in vitro and then subjected to miRNA and messenger RNA microarray analyses. Changes in expression profiles were recorded. Using quantitative polymerase chain reaction (qPCR), flow cytometry, and enzyme-linked immunosorbent assay (ELISA), we confirmed the suppression of… CONTINUE READING