Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger.

@article{Gordon2000GlucoamylasegreenFP,
  title={Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger.},
  author={C L Gordon and Vahid Khalaj and Arthur F. J. Ram and David Brian Archer and Jayne L. Brookman and Anthony P. J. Trinci and David J. Jeenes and John H. Doonan and Brian Wells and Peter J. Punt and Cees A.M.J.J. van den Hondel and G. D. Robson},
  journal={Microbiology},
  year={2000},
  volume={146 ( Pt 2)},
  pages={
          415-26
        }
}
A glucoamylase::green fluorescent protein fusion (GLA::sGFP) was constructed which allows the green fluorescent protein to be used as an in vivo reporter of protein secretion in Aspergillus niger. Two secretory fusions were designed for secretion of GLA::sGFP which employed slightly different lengths of the glucoamylase protein (GLA499 and GLA514). Expression of GLA::sGFP revealed that fluorescence was localized in the hyphal cell walls and septa, and that fluorescence was most intense at… 

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