Gluco-oligosaccharide synthesis by free and immobilized beta-glucosidase.

  title={Gluco-oligosaccharide synthesis by free and immobilized beta-glucosidase.},
  author={C Ravet and Daniel Thomas and Marie Dominique Legoy},
  journal={Biotechnology and bioengineering},
  volume={42 3},
Gluco-oligosaccharides were synthesized through the enzymatic condensation of D-glucose at high concentration using a commercial almond beta-glucosidase. The synthesis reactions were carried out with both free and immobilized enzyme, with or without sorbitol, an efficient depressor of water activity (a(w)) in the presence of different glucose concentrations. The yield and the composition of the gluco-oligosaccharides produced changed with the reaction mixture and the form of the enzyme used… CONTINUE READING

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