Glucagon challenge in the rat: a robust method for the in vivo assessment of Glycogen phosphorlyase inhibitor efficacy.

@article{Loxham2007GlucagonCI,
  title={Glucagon challenge in the rat: a robust method for the in vivo assessment of Glycogen phosphorlyase inhibitor efficacy.},
  author={Susan J G Loxham and Joanne Teague and S. M. Poucher and Joanne De Schoolmeester and Andrew V. Turnbull and Frank Carey},
  journal={Journal of pharmacological and toxicological methods},
  year={2007},
  volume={55 1},
  pages={71-7}
}
INTRODUCTION Glycogen phosphorlyase inhibitors (GPi) act on the glycogenolytic pathway decreasing hepatic glucose output, making them potential candidates for Type 2 diabetes treatment. We established a robust in vivo method to assess GPis efficacy utilising glucagon-stimulated glycogenolysis. METHODS Blood glucose was monitored in both male AP Wistar and AP Zucker rats using tail prick samples pre- and post intraperitoneal or subcutaneous glucagon administration. The effect of glycogen… CONTINUE READING