Glu(332) in the Nicastrin ectodomain is essential for gamma-secretase complex maturation but not for its activity.

@article{ChvezGutirrez2008Glu332IT,
  title={Glu(332) in the Nicastrin ectodomain is essential for gamma-secretase complex maturation but not for its activity.},
  author={Luc{\'i}a Ch{\'a}vez-Guti{\'e}rrez and Alexandra Tolia and Elke Maes and Tingting Li and Philip Chuen Yung Wong and Bart De Strooper},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 29},
  pages={20096-105}
}
The gamma-secretase complex is responsible for the proteolysis of integral membrane proteins. Nicastrin has been proposed to operate as the substrate receptor of the complex with the glutamate 332 (Glu(333) in human) serving as the anionic binding site for the alpha-amino-terminal group of substrates. The putative binding site is located within the aminopeptidase-like domain of Nicastrin. The Glu(332) is proposed to function as the counterpart of the exopeptidase Glu located in the active site… CONTINUE READING