Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis

@article{Kurimoto2007GlobalSC,
  title={Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis},
  author={Kazuki Kurimoto and Y. Yabuta and Y. Ohinata and M. Saitou},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={739-752}
}
  • Kazuki Kurimoto, Y. Yabuta, +1 author M. Saitou
  • Published 2007
  • Medicine, Biology
  • Nature Protocols
  • We describe here a protocol for the representative amplification of global mRNAs from typical single mammalian cells to provide a template for high-density oligonucleotide microarray analysis. A single cell is lysed in a tube without purification and first-strand cDNAs are synthesized using a poly(dT)-tailed primer. Unreacted primer is specifically eliminated by exonuclease treatment and second strands are generated with a second poly(dT)-tailed primer after poly(dA) tailing of the first-strand… CONTINUE READING
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