Global regulation of gene expression in Escherichia coli.

  title={Global regulation of gene expression in Escherichia coli.},
  author={Shuang En Chuang and Donna L. Daniels and Frederick R. Blattner},
  journal={Journal of bacteriology},
  volume={175 7},
Global transcription responses of Escherichia coli to various stimuli or genetic defects were studied by measuring mRNA levels in about 400 segments of the genome. Measuring mRNA levels was done by analyzing hybridization to DNA dot blots made with overlapping lambda clones spanning the genome of E. coli K-12. Conditions examined included isopropyl-beta-D-thiogalactopyranoside (IPTG) induction, heat shock, osmotic shock, starvation for various nutrients, entrance of cells into the stationary… CONTINUE READING


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