Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.

@article{Molina2007GlobalPP,
  title={Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.},
  author={Henrik Molina and David Micha Horn and Ning Tang and S. Mathivanan and Akhilesh Pandey},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 7},
  pages={2199-204}
}
Electron transfer dissociation (ETD) is a recently introduced mass spectrometric technique that provides a more comprehensive coverage of peptide sequences and posttranslational modifications. Here, we evaluated the use of ETD for a global phosphoproteome analysis. In all, we identified a total of 1,435 phosphorylation sites from human embryonic kidney 293T cells, of which 1,141 ( approximately 80%) were not previously described. A detailed comparison of ETD and collision-induced dissociation… CONTINUE READING