Global negative regulation of Streptomyces coelicolor antibiotic synthesis mediated by an absA-encoded putative signal transduction system

@article{Brian1996GlobalNR,
  title={Global negative regulation of Streptomyces coelicolor antibiotic synthesis mediated by an absA-encoded putative signal transduction system},
  author={P. Dilkes Brian and Perry J. Riggle and R. A. dos Santos and Wendy C. Champness},
  journal={Journal of Bacteriology},
  year={1996},
  volume={178},
  pages={3221 - 3231}
}
Streptomycete antibiotic synthesis is coupled to morphological differentiation such that antibiotics are produced as a colony sporulates. Streptomyces coelicolor produces several structurally and genetically distinct antibiotics. The S. coelicolor absA locus was defined by four UV-induced mutations that globally blocked antibiotic biosynthesis without blocking morphological differentiation. We show that the absA locus encodes a putative eubacterial two-component sensor kinase-response regulator… Expand
Transcriptional Regulation of Streptomyces coelicolorPathway-Specific Antibiotic Regulators by the absA andabsB Loci
TLDR
The results establish that the abs genes affect production of antibiotics through regulation of expression of the antibiotic-specific regulatory genes in S. coelicolor through regulating transcript abundance from the biosynthetic and regulatory genes specific for each antibiotic. Expand
Regulation of the Streptomyces coelicolor Calcium-Dependent Antibiotic by absA, Encoding a Cluster-Linked Two-Component System
TLDR
This paper has identified numerous transcriptional start sites within the CDA cluster and shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. Expand
A Streptomyces coelicolor Antibiotic Regulatory Gene, absB, Encodes an RNase III Homolog
TLDR
This study isolated absB-complementing DNA and shows that it encodes the S. coelicolor homolog of RNase III (rnc), indicating that the absB mutant global defect in antibiotic synthesis is due to a deficiency inRNase III. Expand
Genetic suppression analysis of non-antibiotic-producing mutants of the Streptomyces coelicolor absA locus.
The absA locus in Streptomyces coelicolor A3(2) was identified because mutations in it uncoupled sporulation from antibiotic synthesis: absA mutants failed to produce any of the four antibioticsExpand
Genetic and transcriptional analysis of absA, an antibiotic gene cluster‐linked two‐component system that regulates multiple antibiotics in Streptomyces coelicolor
TLDR
A genetic analysis shows that the phosphorylated form of the AbsA2 response regulator (phospho‐AbsA2), generated by the cognate AbsA1 sensor histidine kinase, is required for normal growth phase regulation of antibiotic synthesis. Expand
Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)
TLDR
The analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2 sheds light on the complex mechanism of regulation of antibiotic biosynthesis in Streptomyces coelicolor. Expand
Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)
TLDR
The analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2 sheds light on the complex mechanism of regulation of antibiotic biosynthesis in Streptomyces coelicolor. Expand
Transcriptional activation of the pathway‐specific regulator of the actinorhodin biosynthetic genes in Streptomyces coelicolor
TLDR
Findings provide further evidence that the path leading to the expression of pathway‐specific activators of antibiotic biosynthesis genes in disparate Streptomyces may share evolutionarily conserved components in at least some cases, and suggests that the regulation of streptomycin production, which serves an important paradigm, may be more complex than represented by current models. Expand
Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
TLDR
The data suggest that AbsA2 approximately P inhibits antibiotic production by directly interfering with the expression of pathway-specific regulators of antibiotic biosynthetic gene clusters. Expand
Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor
TLDR
The results support a model in which the phosphorylation state of AbsA2 is determined by the balance of the kinase and phosphatase activities of absA1 and where AbsA 2 approximately P represses antibiotic biosynthetic genes either directly or indirectly. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 80 REFERENCES
abaA, a new pleiotropic regulatory locus for antibiotic production in Streptomyces coelicolor
TLDR
Subcloning experiments revealed that ORFB together with 137 bp downstream of it is responsible for antibiotic overproduction in S. lividans, and abaA constitutes a new locus which, like the afs and abs genes previously described, pleiotropically regulates antibiotic production. Expand
Genetic analysis of absB, a Streptomyces coelicolor locus involved in global antibiotic regulation
TLDR
Genetic analysis of absB mutants has resulted in definition of the absB locus at 5 o'clock on the genetic map, interpreted to mean that the failure of abs B mutants to produce antibiotics results from decreased expression of the antibiotic genes. Expand
Mutations in a new Streptomyces coelicolor locus which globally block antibiotic biosynthesis but not sporulation
TLDR
The phenotype of the absA mutants suggests that all S. coelicolor antibiotic synthesis genes are subject to a common global regulation that is at least in part distinct from sporulation and that absA is a genetic component of the regulatory mechanism. Expand
The level of a transcript required for production of a Streptomyces coelicolor antibiotic is conditionally dependent on a tRNA gene
TLDR
These experiments showed that the undecylprodigiosin deficiency of bldA mutants cannot be accounted for by the presence of TTA codons in the red structural genes, but rather that bld a influences red gene mRNA abundance. Expand
A general role for the lux autoinducer in bacterial cell signalling: control of antibiotic biosynthesis in Erwinia.
TLDR
The first substantive evidence to support the previous postulate that the lux autoinducer, KHL, is widely involved in bacterial signalling is reported, which shows that it acts as a molecular control signal for the expression of genes controlling carbapenem antibiotic biosynthesis. Expand
Additional copies of the actII regulatory gene induce actinorhodin production in pleiotropic bld mutants of Streptomyces coelicolor A3(2)
TLDR
The results indicate that the structural genes for actinorhodin biosynthetic enzymes do not contain targets for the bldA, D, F, G and H gene products, and suggest that the corresponding bld genes control actin orhodin synthesis principally via a single gene in the actII regulatory region. Expand
Spore colour in Streptomyces coelicolor A3(2) involves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics
TLDR
It is proposed that whiE is normally expressed only in the aerial hyphae, where the biosynthetic product is responsible for spore colour, and that six open reading frames show homo‐logy at the level of deduced gene products with genes involved in the synthesis of polyketide antibiotics. Expand
Two developmentally controlled promoters of Streptomyces coelicolor A3(2) that resemble the major class of motility-related promoters in other bacteria
TLDR
In experiments designed to allow isolation of Streptomyces coelicolor promoters that depend on the whiG sporulation gene, an apparent transcription start site was found upstream of an apparent open reading frame (ORF) and just downstream of sequences that resemble consensus features of promoters for motility-related genes in Bacillus subtilis and coliform bacteria. Expand
Identification of Streptomyces coelicolor genes involved in regulation of antibiotic synthesis.
To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of Streptomyces coelicolor's two pigmented antibiotics and found mutant strainsExpand
A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.
TLDR
DNA sequencing of the new determinant, named comP, revealed that the carboxy-terminal domain of the predicted ComP protein is similar in amino acid sequence to that of several sensor members of the bacterial two-component signal-transduction systems. Expand
...
1
2
3
4
5
...