Global metabolomic characterizations of Microcystis spp. highlights clonal diversity in natural bloom-forming populations and expands metabolite structural diversity

  title={Global metabolomic characterizations of Microcystis spp. highlights clonal diversity in natural bloom-forming populations and expands metabolite structural diversity},
  author={Benjamin Marie and S{\'e}verine Le Manach and Charlotte Duval and Arul Marie and Chakib Dj{\'e}diat and Arnaud Catherine and Marc Edery and S{\'e}verine Zirah and C{\'e}cile Bernard},
Cyanobacteria are photosynthetic prokaryotes that are able to synthetize a wild rang of secondary metabolites exhibiting noticeable bioactivity, comprising toxicity. Microcystis represents one of the most common cyanobacteria taxa constituting the intensive blooms that arise nowadays in freshwater ecosystems worldwide. They produce numerous cyanotoxins (toxic metabolites), which are potentially harmful to Human health and aquatic organisms. In order to better understand the variations in… Expand
Draft Genome Sequence of the Toxic Freshwater Microcystis aeruginosa Strain PMC 728.11 (Cyanobacteria, Chroococcales)
The genome sequence contains 276 contigs, consisting of 5,536,025 bp and 5,594 putative protein-coding genes, among which are several biosynthetic gene clusters encoding enzyme complexes involved in the production of various bioactive and toxic metabolites. Expand
Cyanopeptide co-production dynamics beyond microcystins and effects of growth stages and nutrient availability.
The synchronized production of other cyanopeptides along with microcystins emphasizes the need to make them available as reference standards to encourage more studies on their occurrence in blooms, persistence and potential toxicity. Expand
Bioactive Potential of Two Marine Picocyanobacteria Belonging to Cyanobium and Synechococcus Genera
Gas chromatography–mass spectrometry analysis evidenced for the first time the presence of β-N-methylamino-l-alanine and microcystin in these picocyanobacteria, laying the foundation for the production of bioactive compounds of pharmacological interest. Expand
Disruption of fish gut microbiota composition and holobiont’s metabolome by cyanobacterial blooms
Results point to the relevance of accounting for short- and long-term microbiome-related effects in fish ecology, with potential outcomes relevant to conservation biology as well as aquaculture. Expand
Novel cyanobacterial metabolites, cyanopeptolin 1081 and anabaenopeptin 899, isolated from an enrichment culture dominated by Woronichinia naegeliana (Unger) Elenkin
Although these peptides did not exert acute toxic effects at the concentration up to 40 μg ml–1, deterioration of the physiological state of the studied crustaceans, indicated by reduced mobility, was observed and it is important to identify and characterize these compounds. Expand
Degradation of Multiple Peptides by Microcystin-Degrader Paucibacter toxinivorans (2C20)
Although P. toxinivorans was previously identified as a MC-degrader, it also degrades a wide range of peptides under a range of conditions, which could be optimized as a potential biological tool for water treatment. Expand
Degradation of multiple peptides by microcystin-
Since conventional drinking water treatments applied in different countries are inefficient at eliminating potentially toxic cyanobacterial peptides, a number of bacteria have been studied as anExpand
New Micropeptins with Anti-Neuroinflammatory Activity Isolated from a Cyanobacterial Bloom
Two new micropeptins are isolated and characterized along with the previously characterized micropeptin 996 and showed potency in anti-neuroinflammatory assays using BV-2 mouse microglial cells, showing a 50% reduction in inflammation in a range from 1 to 10 μM. Expand
Semi-automated classification of colonial Microcystis by FlowCAM imaging flow cytometry in mesocosm experiment reveals high heterogeneity during seasonal bloom
It is concluded that automatic methods not only allow a performance level of human taxonomist, and thus be a valuable time-saving tool in the routine-like identification of colonial phytoplankton taxa, but also can be applied to increase temporal and spatial resolution of the study. Expand


Comparative Metagenomics of Toxic Freshwater Cyanobacteria Bloom Communities on Two Continents
Despite variation in the phylogenetic assignments of bloom-associated organisms, the functional potential of bloom members remained relatively constant between systems, and this pattern was particularly noticeable in the genomic contribution of nitrogen assimilation genes. Expand
Diversity and distribution of Microcystis (Cyanobacteria) oligopeptide chemotypes from natural communities studied by single-colony mass spectrometry.
The in situ diversity of peptides and chemotypes in Microcystis communities from lakes in and around Berlin, Germany is studied by direct analysis of individual colonies by MALDI-TOF mass spectrometry, highlighting the immense metabolic potential and diversity of this genus. Expand
Changes in secondary metabolic profiles of Microcystis aeruginosa strains in response to intraspecific interactions.
New insights into the factors that regulate the production of MC and other secondary metabolites in cyanob bacteria are provided, and interchangeable or complementary functions allowing bloom-forming cyanobacteria to efficiently colonize and dominate in fluctuating aquatic environments are suggested. Expand
Diversity of Microcystis aeruginosa isolates (Chroococcales, Cyanobacteria) from East-African water bodies
Only 4 of the 24 isolated strains from East Africa were found to produce microcystins, while oligopeptides belonging to the aeruginosin and cyanopeptolin class were detected in most strains. Expand
A Tribute to Disorder in the Genome of the Bloom-Forming Freshwater Cyanobacterium Microcystis aeruginosa
The genomes of Microcystis aeruginosa and Prochlorococcus are sequenced, showing that they are characterized by having almost opposite evolutionary strategies, both of which have led to ecological success in their respective environments. Expand
Improving the coverage of the cyanobacterial phylum using diversity-driven genome sequencing
This phylum-wide study highlights the benefits of diversity-driven genome sequencing, identifying more than 21,000 cyanobacterial proteins with no detectable similarity to known proteins, and foregrounds the diversity of light-harvesting proteins and gene clusters for secondary metabolite biosynthesis. Expand
Hepatotoxic Cyanobacteria: A Review of the Biological Importance of Microcystins in Freshwater Environments
Cyanobacteria play vital roles in aquatic food webs, yet production, accumulation, and toxicity patterns of MCs within aquaticFood webs remain obscure. Expand
Role of bacteria in the production and degradation of Microcystis cyanopeptides
Investigation of the bacterial genetic diversity adapted to the PCC7806 strain revealed that a natural bacterial community, dominated by Alpha‐proteobacteria, was able to degrade a wide panel of structurally varying cyclic cyanopeptides. Expand
Unique Biosynthetic Pathway in Bloom-Forming Cyanobacterial Genus Microcystis Jointly Assembles Cytotoxic Aeruginoguanidines and Microguanidines.
Aeruginoguanidines and microguanidines were found to be as widespread as the hepatotoxic microcystins, but the occurrence of both toxin families appeared to be mutually exclusive. Expand
A review of the global ecology, genomics, and biogeography of the toxic cyanobacterium, Microcystis spp.
The ability of Microcystis assemblages to minimize their mortality losses by resisting grazing by zooplankton and bivalves, as well as viral lysis, and discuss factors facilitating assemblage resilience are highlighted. Expand